读书报告揭秘根际微生物中的抗病性细菌演示课件.pptVIP

Deciphering the Rhizosphere Microbiomefor Disease-Suppressive Bacteria揭秘根际微生物中的抗病性细菌;OUTLINE;Introduction The Aim of This Study; For most disease-suppressive soils, the microbes and mechanisms involved in pathogen control are unknown. 对于绝大多数的抗病性土壤而言，其调控病原菌的微生物种类及其机制还不清楚。 ;Similar to other eukaryotes（真核生物）, plants and their microbiomes can be viewed as “superorganisms” in which the plant relies, in part, on the soil microbiotic for specific functions and traits. 与其它真核生物相似，植物与它们根际的微生物被称为“超个体”，由于植物的生长部分依赖于土壤微生物特殊的功能和特性。;In return, plants exude up to 21% of their photosynthetically fixed carbon in the root-soil interface, thereby feeding the microbial communities and influencing their activity and diversity. 作为反馈，植物输出其光合作用固定的碳的21%进入根土界面，以此来供养微生物群落，并影响它们的活性和多样性。;Materials Methods;Disease suppressiveness was determined for various treatments: suppressive soil (S) conducive soil (C) conducive soil amended with 10% (w/w) of suppressive soil (CS) suppressive soil heat-treated at 50℃ for 1 hour (S50) suppressive soil heat-treated at 80℃ for 1 hour (S80) ;I- Experimental design 1 Sugar beet grown in the presence of R. solani (N=4). 2 Sugar beet grown in the absence of R. solani (N=4). * Treatments selected for PhyloChip analysis.; ;III - Isolation of specific bacterial taxa targeted by PhyloChip analysis ;IV - Genes and pathways involved in disease suppression Genetic, bioinformatic, and functional analyses Mutagenesis（引发突变） Genome library constructions Sequencing Signature-sequence-based predictions in vivo bioassays. ;Results;PhyloChip analysis A total of 33,346 bacterial and archaeal（古细菌） operational taxonomic units (OTUs) were detected in the rhizosphere microbiome . ;The relative abundance of several bacterial taxa is a more important indicator of disease suppression than the exclusive presence of specific bacterial taxa.;;The γ- and β-Proteobacteria(变形菌门）and the Firmicutes（厚壁菌门）were identified as the most dynamic ta