[医学]生理第三章 生命基本特征与神经肌肉一般特性.pptVIP

第三章 人体的基本生理功能 药学医学基础实验中心 生理教研室 Dqlcpu@163.com Patch Clamping The technique of patch clamping is used to study ion channel activity. A narrow bore micropipet may be pushed up against a cell or vesicle, and then pulled back, capturing a fragment of membrane across the pipet tip. Patch Clamping A voltage is imposed between an electrode inside the patch pipet and a reference electrode in contact with surrounding solution. Current is carried by ions flowing through the membrane. If a membrane patch contains a single channel with 2 conformational states, the current will fluctuate between 2 lev