肉鸡发生腺胃炎后炎症因子的变化-中国畜牧业协会.pdfVIP

实验研究 肉鸡发生腺胃炎后炎症因子的变化※ ※※ ※※※ 张亚莉 ，刘文强 （聊城大学农学院，山东聊城 252059 ） 摘 要：鸡传染性腺胃炎是一种由多种致病因素协同作用引起的一种传染性慢性消耗性疾病，给 养禽业带来严重的损失。观察了该病的发病过程，对于不同日龄自然感染鸡进行腺胃肉眼病变计分， 发现患病鸡在 21d 时计分最高：腺胃表面有明显的坏死灶，腺胃乳头出血，脱落甚至消失，法氏囊和 胸腺已经发生萎缩并且胰腺坏死。采用ELISA 对21d 的10 只817 肉杂鸡进行测定表明：与对照组相 比，患病鸡IL-1 分泌量增加，但差异不显著；IL-6、IL-8、TNF- ɑ的分泌量分别达到76.9337±7.2334 ng/L 、 62.2063±7.8693 ng/L 和 134.5988±18.6497，明显高于对照组，且差异显著。SIgA 分泌量为 19.1875±1.9768 ng/L，明显高于对照组，差异极显著。前文已描述了在此病例过程中，IL-2 和IFN-γ 含 量显著减少。这些炎性细胞因子的变化表明鸡群感染传染性腺胃炎后出现了免疫抑制现象，同时发生 了全身性/局灶性炎症，对疾病的治疗造成了极大的困难。这种变化有助于早期诊断该病。 关键词：肉杂鸡；传染性腺胃炎；炎症因子 Changes of inflammatory factors after infection of proventriculitis in hybrid broilers ZHANG Yali, LIU Wenqiang* (College of agricultural of Liaocheng University ，Liaocheng,Shandong province,252059) Abstract: Broiler proventriculitis is an infectious chronic wasting disease by a variety of pathogenic factors synergistic effect, causing serious losses to poultry industry. The pathogenesis of the cases is observed ： proventriculus gross lesions is counted to different ages of naturally infected 817 hybrid broilers (n=10, take the average value)according to the method of Keyburn. The result suggest the highest point in the 2 1d ages：obvious necrosis on the surface of the glandular stomach ，bleeding of the glandular stomach, and even disappeared ， the bursa and thymus have been contracted and pancreatic necrosis. The cytokine concentration of serum determinate by ELISA of 10 chickens of 21d ages suggested：compared with the control group, the amount of IL-1 increased, but there was no significant difference between the