基于OLED的聚丙烯生物光盘传感器.docxVIP

外文资料原文OLED-Polypropylene Bio-CD SensorSrikanth Vengasandra, Yuankun Cai, David Grewell, Joseph Shinar, and Ruth Shinarc Department of Agricultural Biosystems EngineeringAmes Laboratory - USDOE and Department of Physics AstronomyMicroelectronicsResearchCenter and Department of Electrical and Computer EngineeringIowaStateUniversity, Ames, IA50011, U.S.AABSTRACTWith the goal of developing microfluidic platforms for sensing applications, flash-free micro patterns were embossed in polypropylene surfaces with ultrasonic heating for a biosensing application. The embossed features were designed to act as reservoirs, valves, and reaction chambers to allow, in combination with a compact sensing platform, the monitoring of analyte levels using a standard PC-CD player. To generate the compact sensor, as an example, we chose the photoluminescence (PL)-based detection of lactate and glucose using an OLED-based sensing platform. Once embossed, the surface energy of the plastic substrate was chemically modified to make it hydrophilic. Reagents, placed in separate reservoirs, were directed through burst valves towards a reaction chamber via CD rotation. Lactate or glucose were monitored by measuring the effect of the related dissolved oxygen level on the PL decay time of an oxygen-sensitive dye, following analyte oxidation catalyzed by a suitable specific oxidase enzyme. The results demonstrate the potential of integrating OLEDs as excitation sources in PL-based sensors with microfluidic CD- based platforms, including for simultaneous multiple analyses.Keywords: Organic light-emitting diode, OLED, lab-on-a-CD, glucose sensor, lactate sensor1. INTRODUCTIONBiomedical micro electromechanical system (MEMS)-based sensing platforms fabricated on plastic substrates have the potential of e.g., being low cost, disposable, cross-contamination free, and sensitive. Additionally, such sensors show promise for high throughput and multianalyte detection. The advantages of such a lab-on-CD-based