[化学]外文文献和对应翻译：Ligand-Exchange Capillary Electrophoresis配体交换毛细管电泳.docVIP

英文文献 Ligand-Exchange Capillary Electrophoresis Abstract: The fundamentals of ligand-exchange capillary electrophoresis are discussed, and the potential of the method in solving major problems, such as the separation of enantiomers and the determination of biologically active compounds poorly absorbing in the UV region (sugars, amines, and amino acids) is considered. Keywords: ligand exchange, capillary electrophoresis, complexation, biologically active substances. The separation methods based on ligand exchange are sensitive to the spatial structure of the analyte molecules, which enables the separation of geometric and optical isomers. The method of ligand-exchange chromatography was used in 1968 by Davankov and Rogozhin for separating amino acid enantiomers [1]. Polystyrene with immobilized L-proline was used as a stationary phase, while the ions of copper(II) and other transition metals were added into the eluent as complexants. Later, silica-based adsorbents with immobilized optically active ligands came into use [2]. One of the promising electrophoretic versions for analyzing complex mixture of natural compounds is ligand-exchange capillary electrophoresis (LECE) [3-19], whose closest analogue is ligand-exchange chromatography [1]. Ligand-exchange capillary electrophoresis is mainly used for separating optical isomers of amino and hydroxy acids; in fact, this is a chiral version of LECE [3-21]. Chiral separation in LECE, as well as in ligand-exchange chromatography, is based on the formation of diastereomeric complexes with a metal cation between the ligand of a chiral selector (L-Sel) and the analyte enantiomers (L-A and D-A) [5]: The formed complexes of analyte?Mn+ are different in stability, which causes the difference in the electrophoretic mobilities of the enantiomers : where and are the molar fractions and and are the electrophoretic mobilities of the unbound analyte (f) and the corresponding complex (c). Factors affecting the analyte separation. Wea