基因测序与组装.pptVIP

OUTLINE DNA sequencing Genome Assembly 2012 Published Genomes What is DNA sequencing? DNA sequencing is to determine the order of the nucleotide bases in a molecule of DNA. First generation sequencer: Chain-termination methods Next generation sequencer(Illumina/Roche): High-throughput sequencing Third generation sequencer(PacBio): Single molecule Chain-termination methods Chain-termination methods Illumina sequencing 454 sequencing What is genome assembly? Sequence assembly refers to aligning and merging fragments to a much longer DNA sequence in order to reconstruc