一个普通小麦Trx超家族新基因TaNRX的克隆与抗旱相关标记开发课件.pdf

作物学报 ACTA AGRONOMICA SINICA 2014, 40(1): 29−36 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2014.00029 一个普通小麦Trx 超家族新基因TaNRX 的克隆与抗旱相关标记开发 * 张 帆 蒋 雷 鞠丽萍 金秀锋 王 轩 张晓科 王宏礼 付晓洁 / , 712100 : RACE , (Trx) (TaNRX) cDNA (GenBank KC890769), 2015 bp, 1734 bp; 577 , 63.79 kD, 3 Trx , 2 Cys-X1-X2-Cys , TaNRX 5B , 4 3 , 1 4 , 150 () TaNRX 2 , TaNRX-a TaNRX-b; TaNRX-a () TaNRX-b (P0.01), : ; ; TaNRX; Cloning a Novel Gene TaNRX of Trx Superfamily and Developing Its Molecular Markers Related to Drought Resistance in Common Wheat * ZHANG Fan, JIANG Lei, JU Li-Ping, JIN Xiu-Feng, WANG Xuan, ZHANG Xiao-Ke , WANG Hong-Li, and FU Xiao-Jie College of Agronomy, Northwest AF University / Yangling Subcenter of National Wheat Improvement Center, Yangling 712100, China Abstract: Molecular markers associated with genes for drought resistance play an important role in wheat breeding aiming at improvement of drought resistance. In this study, we obtained the full-length cDNA of a novel gene of thioredoxin (Trx) super- family, TaNRX (GenBank accession number KC890769), from common wheat (Triticum aestivum L.) using the protocol of gene homology cloning, electronic cloning, RACE, and bioinformatics analysis. The open reading frame (ORF) of TaNRX is 1734 bp in le