门控-弹簧模型及其在果蝇机械力信号转导中的分子基础-生理学报.PDFVIP

生理学报Acta Physiologica Sinica , February 25, 2016, 68(1): 87–97 87 DOI: 10.13294/j.aps.2016.0001 综 述 “门控 - 弹簧”模型及其在果蝇机械力信号转导中的分子基础 梁鑫* 清华大学生命科学学院，北京 100084 ；清华大学-北京大学生命科学联合中心，北京 100084 摘 要：机械力是生物体生命活动中普遍存在的一种物理刺激，高等生物对机械力的感受是听觉、触觉、压觉以及平衡觉等 重要生理过程的生物学基础。生物体中的感受器细胞对机械力信号的转导是上述感受的关键，这一信号转导过程可将细胞 - (“Gating-Spring” model) 外机械力刺激转换为细胞的电信号或者化学信号。“门控 弹簧”模型 在细胞水平定量地描述了机械 ( ) 力信号转导的原理，但是其分子基础 包括机械力信号转导通路的分子组分，结构基础以及力学原理 依然有待探索。近年 - 来，人们以果蝇为模式生物对上述问题进行了系统的研究，取得了重要的进展。本文对“门控 弹簧”理论进行了概述，并 对在果蝇系统中取得的研究进展以及有待解决的问题进行综述和展望。 关键词：果蝇；机械力信号转导；“门控-弹簧”模型；力敏感离子通道；细胞骨架 中图分类号：Q274 “Gating-Spring” model and molecular basis of mechanotransduction in Drosophila melanogaster * LIANG Xin School of Life Sciences, Tsinghua University, Beijing 100084, China; Tsinghua-Peking Joint Center for Life Sciences, Beijing 100084, China Abstract: The sense of mechanical stimuli (e.g. force or deformation) in the environment underlies several important physiological processes, for example the perception of sound, touch, pain and acceleration. The key step in mechanosensation is to convert the extracellular mechanical stimuli into cellular electrical or chemical signals. This process is termed as mechanotransduction. Based on mechanical and electrophysiological measurements, “Gating-Spring” theory was proposed as a general model to describe the cell biological mechanism of mechanotransduction. However, despite efforts made in several model organisms, the molecular basis of the “Gating-Spring” model remains elusive. In recent years, several key progresses have been made using the mechanoreceptors of Drosophila melanogaste