SNP研究进展及在家畜育种中的作用.pptVIP

Two large undecameric N-RNA rings pack head to head in the crystal asymmetric unit. Each ring has an outer diameter of 160 ),an inner diameter of 60 ), and a height of 74 ) The N protomer consists of two main domains, which contact nine nucleotides of single-stranded RNA, as predicted (14), that are occluded in the center of the ring. The N-terminal core domain (NTD; residues 32 to 233) folds into a helical arrangement composed of 6 helices connected by large loops. The C-terminal core domain (CTD; residues 236 to 356 and 396 to 450) is composed of 11 helices joined by tighter loops (Figs. 1C and fig. S1). Two regions in N (NTD: 105 to 118 and CTD: 376 to 397) are presumably flexible, because they are absent in the structure (Fig. 1C and fig. S1). The closest contact between the two jaws is between NTD residue N157 and CTD residue P435 (9 to 10 ), The RNA strand is twisted clockwise in an irregular left-handed helix along the inner perimeter of the ring within the continuous cleft made up by the NTD (top) and CTD (bottom) interface in the ring structure There are no significant interactions between the NTDs within the ring structure (Fig. 1B). Their position is mainly determined by the connection to the CTD, the bound RNA, and extensive crystal contacts between NTDs of the two rings in the crystal asymmetric unit. In contrast, the CTDs share a large interactionsurface (2700 )2 total buried surface) (Fig. 1B) that is determined by hydrophobic and van der Waals contacts as well as multiple polar in- teractions (total of 15) involving many main chain contacts (total of nine contacts). * *