H2O2促进中性粒细胞对人脐静脉血管内皮细胞的黏附和损伤-生理学报.PDFVIP

412 生理学报Acta Physiologica Sinica , August 25, 2012, 64(4): 412–416 研究论文 H O 促进中性粒细胞对人脐静脉血管内皮细胞的黏附和损伤 2 2 * 钱中清 ，何小燕，吕静竹，汪洪涛，方芳 蚌埠医学院免疫学教研室，安徽省感染免疫重点实验室，蚌埠 233003 摘 要：本文旨在探讨H2O2对中性粒细胞(polymorphonuclear neutrophils, PMNs) 黏附和损伤人脐静脉血管内皮细胞(human umbilical vein endothelial cells, HUVECs) 的促进作用。应用相差显微镜和扫描电镜，以未经H2O2处理的PMNs与HUVECs共培 养为对照，观察H2O2预处理的PMNs对HUVECs 的黏附和损伤，并计数其黏附率；应用流式细胞仪检测H2O2 预处理的PMNs 对HUVECs表达细胞间黏附分子1 (intercellular adhesion molecule 1, ICAM-1)和Apo2.7水平的影响。结果显示，外源性H2O2预 处理的PMNs与HUVECs共培养后，相差显微镜下可见圆形PMNs黏附于HUVECs表面，HUVECs细胞形态呈圆形或椭圆形， 体积变小；扫描电镜下，细胞微绒毛变短或消失，部分细胞胞膜结构破坏，甚至有细胞碎片形成；H2O2预处理的PMNs对 HUVECs 的黏附率为(57.74 ± 9.18)%，显著高于对照组[(23.12 ± 6.43)%, P 0.01, n = 8] ；HUVECs表达黏附分子ICAM-1及凋 亡相关分子Apo2.7 的百分率分别为(44.69 ± 1.52)%和(39.29 ± 1.81)% ，显著高于对照组[(21.79 ± 1.43)%和(9.79 ± 1.43)% ](P 0.01, n = 8) H O PMNs HUVECs 。以上结果表明 可促进 对 的黏附和损伤。 2 2 关键词：过氧化氢；中性粒细胞；血管内皮细胞；细胞间黏附分子1 中图分类号：R331.1 H O promotes neutrophil adherence and injury of human umbilical vein 2 2 endothelial cells * QIAN Zhong-Qing , HE Xiao-Yan, LV Jing-Zhu, WANG Hong-Tao, FANG Fang Key Laboratory of Anhui Province for Infection and Immunology, Department of Immunology, Bengbu Medical College, Bengbu 233003, China Abstract: To explore the role of hydrogen peroxide (H O ) in promoting polymorphonuclear neutrophils adherence and injury of hu- 2 2 man umbilical vein endothelial cells (HUVECs), the ordinary optical microscope and scanning electron microscopy were used to ob- serve the adherence and injury after HUVECs co-cultured with neutrophils pretreated by extracellular H O (HUVECs and neutrophils