毕业设计（论文）-瓜实蝇致病菌粘质沙雷氏菌的分离与鉴定精选.docxVIP

海 南 大 学毕 业 论 文（设计）题 目：瓜实蝇致病菌粘质沙雷氏菌的分离与鉴定 学 号： 姓 名： 年 级： 2010 级 学 院： 环境与植物保护学院 系 别： 植物保护 专 业： 植物保护（农药方向） 指导教师： 完成日期： 2014 年 5 月 1 日 摘 要从瓜实蝇虫尸上分离出的一株产紫红色色素的细菌BC-hn212，染病死亡后的瓜实蝇成虫腹部明显发红。采用形态学、生理生化方法对其进行初步鉴定；并提取基因组DNA，16S rDNA基因序列分析。该菌株菌落圆形隆起，光滑粘稠，有轻微异味；24h后菌落颜色加深、气味变为恶臭，显微镜下观察为球形或杆状菌；革兰氏染色阴性，甲基红为阳性，V.P阳性，淀粉水解实验阳性，果聚糖形成阴性，H2S产生阴性，明胶水解阳性，过氧化氢酶阳性，氧化酶阴性。根据形态学于生理生化方法的鉴定结果初步判定为沙雷氏菌属。根据16S rDNA基因序列分析结果，可以确定此株菌株为粘质沙雷氏菌。本实验初步证明粘质沙雷氏菌对于瓜实蝇具有较强致病能力，为进一步研究粘质沙雷氏菌对于昆虫存在致病性提供依据。关键词：瓜实蝇；粘质沙雷氏菌；分离与鉴定AbstractA strain isolated from the purple pigment bacteria (2-1-1), after expanding training for health melon fly 3 to 5 days after dying bacteria, melon fly abdominal became red and melon fly died. Then to isolate the bacteria, the morphological, physiological and biochemical method carries on the preliminary appraisal; Extraction of genomic DNA, using 16 s rRNA universal primers, PCR amplification of 16 s rDNA gene fragments, through multiple sequence alignment and homology analysis, constructing phylogenetic trees, serratia bacteria has the highest homology.The circular uplift mutants were smooth viscous and slight odor; After 24h the colony color deepened, and it smell like a mess. Using Microscope to observe, it looked spherical or rod-shaped bacteria. Gram was negative, and methyl red was positive, and Valerie plame was positive, and starch hydrolysis experiments is positive, and fructan formation was negative, and H2S produced negative, and gelatin hydrolysate was positive, and catalase was positive, and oxidase was negative. According to the result of morphology in identification of physiological and biochemical methods, we determine initial serratia. Then we use 16SrDNA gene sequence analysis,. According to the compared results, we certain that this strain of bacteria is serratia marcescens.The experiment preliminary proves that serratia marcescens has strong ability of pathogenic for melon fly, for the further study of serratia marcescens, it provides evidence to prove that serrat