一个新巨胚等位基因的鉴定及性状考察与表达分析课件.docVIP

一个新巨胚等位基因的鉴定及性状考察与表达分析 平宝哲，时亚琼，谢米雪，张鑫，王鑫，张涛，李建粤* （上海师范大学 生命与环境科学学院植物种质资源开发中心，上海200234） 摘要：“超2-10”水稻“上师大5号”巨胚水稻胚重胚与糙米量比基因（GE）序列结果显示，“上师大5号”的胚重胚与糙米量比都极显著大于“超2-10”“超2-10”的胚重接近或大于一些已报道的巨胚水稻“上师大5号”巨胚表型由一对隐性基因控制韩国巨胚稻“M-2-565-11-3-B(ge)”“超2-10”、“上师大5号”和“M-2-565-11-3-B(ge)”种水稻各自具有一个新的GE等位基因。“上师大5号”水稻中，启动子及编码链都有突变的GE等位基因（gec）GE等位基因呈持续下降的表达模式。本研究首次报道了突变在启动子、突变同时发生在启动子及编码链种新型巨胚等位基因。 关键词：巨胚基因“上师大5号”“超2-10”“M-2-565-11-3-B(ge)”；新等位基因表达分析 Molecular Characterization, Phenotype Investigation and Expression Analysis of a New Giant Embryo Allele PING Baozhe, SHI Yaqiong, XIE Mixue, ZHANG Xin, WAN Xin, ZHANG Tao, LI Jianyue* （Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, People’s Republic of China） Abstract: In this paper, we use “Chao2-10” and “Shangshida No. 5” as materials. The embryo weight and ratio of embryo to brown rice of “Shangshida No. 5” were compared with those of “Chao2-10”. Then we did the genetic analysis of giant embryo phenotype. Giant embryo gene (GE) of “Shangshida No. 5”, “Chao2-10” and Korea giant embryo rice“M-2-565-11-3-B (ge)” were cloned to analyse their sequence. The expression levels of GE gene in “Shangshida No. 5”, “Chao2-10” were analysed by Real time-PCR with the “Nipponbare” as a control. The results were as follows: (1) Both embryo weight and ratio of embryo weight to brown rice weight of “Shangshida No. 5” was significantly larger than these of “Chao2-10” (P0.01). However, embryo weight of the “Chao2-10” is close to or more than some of the giant embryo rice cultivars reported previously; (2) Giant embryo phenotype of “Shangshida No. 5” is controlled by a single recessive gene, which is same to “M-2-565-11-3-B(ge)”; (3) GE gene in these three cultivars is a new GE allele: “Chao2-10” has a mutation (gep) in the promoter; “Shangshida No. 5” has mutations (gec) both in the promoter and CDS;“M-2-565-11-3-B(ge) only has a mutation (geh) in CDS; (4) The expression lev