对过敏性疾病发病机制的几点新认识教材幻灯片.pptVIP

5. 另一项研究发现哮喘小鼠气道IL-17主要来源于肺泡内的巨噬细胞而不 是Th17细胞,气道内IL-17的表达可以被肥大细胞介质上调,但被IL-10 下调; 6. 特应性皮炎患者的外周血Th17细胞比例增加,且与疾病的严重程度相关, IL-17可促进角质细胞表达和分泌IL-8、TNFα、CXCL10、GM-CSF; 7. 动物试验发现小鼠皮下注射卵白蛋白后可产生特应性皮炎患者类似的皮肤 表现,同时伴有分泌IL-17的T细胞计数增加和IL-17血清水平的增加。 8. 但是用IL-17a和IL-17f基因敲除的小鼠进行研究时发现,IL-17在皮肤超敏 反应中仅起很小的作用; 9. Th17细胞和IL-17在其他过敏性疾病如过敏性鼻炎、过敏性结膜炎和食物 过敏发病机制中的作用还不明确。有的研究检测到过敏性鼻炎患者鼻分 泌物中的IL-17增加。另一些研究则发现过敏性鼻炎病变皮肤缺乏Th17 细胞。 Table 1. Number of IL-29 containing cells in foreskin, colon, tonsil and lung tissues stained by anti-human tryptase antibody G3 and anti-human IL-29 antibody SC1 with a double labeling immunohistochemical technique Tissue Positive stained cells G3/mm2 SC1/mm2 G3 and SC1/mm2 % G3 positive cells expressing IL-29 Foreskin 3.0 (0.7 – 5.8) 2.5 (0.6 – 5.1) 58.7 (27 – 120) *? 95 (94 – 97) Colon 5.0 (0.5 – 15.8) 5.9 (0.4 – 28.8) 70.4 (55.8 – 121) *? 95 (78 – 99) Tonsil 4.5 (0.7 – 6.5) 2.2 (0.2 – 8.2) 34.7 (8.6 – 129) *? 96.5 (92 – 99) Lung 10.2 (6.0 – 14.7) 2.2 (0.8 – 8.0) 18.4 (11.1 – 37.3) *? 88 (82 – 99) Data shown are the median (range) value from 6 different donors. Positively stained cells in at least 30 fields (? 400 magnification) of each section were counted. ﹡ P 0.05 in comparison with the cells stained by G3 antibody alone. ? P 0.05 in comparison with the cells stained by SC1 antibody alone (paired Mann-Whitney U-test). 其它能够诱导肥大细胞募集的肥大细胞分泌产物 1、组胺通过H4受体受体诱导肥大细胞的聚集:Hofstra CL, et al. Histamine H4 receptor mediates chemotaxis and calcium mobilization of mast cells. J Pharmacol Exp Ther, 2003, 305(3):1212-1221. ; 2、 五羟色胺通过其受体诱导肥大细胞的聚集:Kushnir-Sukhov NM, et al. 5-hydroxytryptamine induces mast cell adhesion and migration. J Immunol, 2006, 177(9):6422-6432. ; 3、 金属蛋白酶9 能够诱导肥大细胞祖细胞在组织中聚集:Di Girolamo N, et al. Human mast cell-derived gelatinase B (matrix metalloproteinase-9) is regulated by inflammatory cytokines: role in cell migration. J Immunol, 2006, 177(4):2638-2650. ;