医学新知导论教材幻灯片.pptVIP

醫學新知導論Mass Spectrometry in Biotechnology/Proteomics;Outline;The Five Mass Spectrometry Nobel Prize Pioneers;WHAT IS A “MASS SPECTROMETER ”...?;The black box problem…...;“A mass spectrometer measures the molecular weight….”;Data Processing;hn;HPLC - MS;Electro-spray, ESI (電灑法);HPLC -MS;;HPLC- MS;ESI and APCI differ in…;HPLC -MS;;HPLC -MS;MALDI Time-of-flight (TOF) MS;Ion Trap;Fourier-transform Ion Cyclotron Resonance (FT-ICR);Single MS Analyzer;HPLC-MS/MS Triple Quadrupole;MS/MS Scan Functions;Product Ion Scanning; Parked on Precursor ion;Precursor Ion Scanning;;;Proteomics Introduction Mass spectrometry in proteomics Protein identification Quantitative proteomics Post-translational modification;Proteome : In 1993, the term “Proteome”, by Marc Wilkins and Keith Williams, was referred to the systematic identification of the entire protein population expressed by a genome or by a cell or tissue type. Proteomics : The subject of proteomic analysis of the proteome (PROTEin complement expressed by a genOME or by a cell or tissue type). ;Definition of Proteomics;蛋白體的發展里程碑;Why analyze gene expression at the protein level?;Y;A polypeptide can fold to generate a particular three-dimensional structure specified by its amino acid sequence. The structural description of proteins is described in terms of four levels of organization.;MS-based Proteomics;The general Property Differences between DNA and Protein;Goal of Genomics and Proteomics;Protein Extract;Excise spot; wash; digest;Peptide-Mass Fingerprinting (PMF);Advantages Disadvantages of 2D gel Approach;Limitation of 2-D Gel Based Proteomics Platform;Protein Detection Methods;High-Performance Liquid Chromatography;Multi Dimensional Liquid Chromatography;Size Exclusion;Affinity Chromatography;Ion Exchange Chromatography;Ion Exchange Chromatography;Hydrophobic Interaction;General approaches;Protein Identification by Peptide Fragment Fingerprinting;Sample Ions;MALDI-TOF/TOF;;nanoRPLC-ESI-Tandem MS;;;N- and C-terminal Pept