Ultraviolet-Visible Spectroscopy[紫外可见分光](PPT-30).pptVIP

Ultraviolet-Visible Spectroscopy Introduction to UV-Visible Absorption spectroscopy from 160 nm to 780 nm Measurement of transmittance Conversion to absorbance A=-logT=ebc Measurement of transmittance and absorbance Beer’s law Noise Instrumentation Measurement Scattering of light Refraction at interfaces Scatter in solution Large molecules Air bubbles Normalized by comparison to reference cell Contains only solvent Measurement for transmittance is compared to results from reference cell Beer’s Law Based on absorption of light by a sample dPx/Px=dS/S dS/S=ratio of absorbance area to total area