结构生物学研究组.pptVIP

ChIP-on-chip ChIP Labeling pool of DNA fragments. Hybridization of DNA onto microarrays featuring 60-mer oligonucleotide probes. Major types of array platforms NimbleGen Arrays: tiling arrays, promoter arrays, whole genome arrays. (/products/chip/index.html) Agilent Arrays: promoter arrays, whole genome arrays. (/Scripts/Phome.asp) Affymetrix Arrays: tiling arrays, Chr21,22 arrays, whole genome arrays. (/index.affx) Measurement of intensity of probes on the array The hybridized arrays were scanned on an Axon GenePix 4000B scanner (Axon Instruments Inc.) at wavelengths of 532 nm f