lmmunohistochemical Localization of Choline Acetyltransferase…胆碱乙酰转移酶的免疫组化定位.pdfVIP

The Journal of Neuroscience, May 1967, 7(5): 1361-1369 lmmunohistochemical Localization of Choline Acetyltransferase During Development and in Chats Mutants of Drosophila melanogaster Michael G. Gorczyca and Jeffrey C. Hall Department of Biology, Brandeis University, Waltham, Massachusetts 02254 The distribution of choline acetyltransferase (CAT) in the nervous systems(Treheme, 1966;Sanesand Hildebrand, 1976). nervous system of Drosophila melanogaster was deter- Also, oc-bungarotoxin(LU-BTX)binding sitesarepresenton what mined by indirect immunohistochemical procedures using a seemto be second-orderneuronsthat receive cholinergic input monoclonal antibody specific to the enzyme. Immunoreac- (Saneset al., 1977;Satelleet al., 1980).In the cockroach, ion- tivity was first detected in the nervous system of 16 hr em- tophoresisof ACh or of cholinergic agonistsproduced activity bryos, and increased considerably by the end of embryo- in cells postsynapticto the mechanoreceptorafferents(Callec, genesis. Neuropil was preferentially stained, though cell 1976),and AChE inhibitors led to increasedactivity (Satelleet bodies could also be observed. Staining was prominent in al., 1980). In addition, both nicotine and (u-BTX have been the CNS of all 3 larval instars but decreased substantially shown to block theseapparent synaptic responses(Harrow et during the mid-pupal stage. Prior