Molecular evidence of Tomato yellow leaf curl virus Sicily…番茄黄叶卷曲病毒西西里的分子证据.pdfVIP

Phytopathol. Mediterr. (2004) 43, 177–186 Molecular evidence of Tomato yellow leaf curl virus-Sicily spreading on tomato, pepper and bean in Tunisia FATEN GORSANE1, 3, IMENE FEKIH-HASSEN 1, MARK K. NAKHLA2 , HATEM FAKHFAKH 1, 3, DOUGLAS P. MAXWELL2 and MOHAMED MARRAKCHI 1 1Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, Campus Universitaire, 2092, Elmanar, Tunis, Tunisie 2Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI 153706 USA 3Faculté des Sciences de Bizerte, Laboratory of Genetics, 7021, Zarzouna, Bizerte, Tunisia Summary. Unusual symptoms including yellowing, stunting, curling, crumpling and plant size reduction were ob- served in tomato fields and green houses in Tunisia. These symptoms, generally associated with the Tomato yellow leaf curl virus (TYLCV) complex, have become increasingly common in recent years. In order to ascertain the molecu- lar characteristics of Tunisian isolates by PCR, both the coat protein gene and the intergenic region of eleven isolates were amplified using specific primers, and sequenced. The PCR procedure also allowed the amplification of viral DNA fragments using a bean total DNA as a template. Phylogenetic analysis suggested that these Tunisian isolates clustered with a Sicilian isolate of TYLCSV-Sic. This is the first report of the involvement of this viral species in Phaseolus vulgaris disease. Key words: TYLCSV, surveys, CP gene, IR region, sequence variability. Introduction twinned isometric particles (Harrisson, 1985).