Studies on the biocidal and cell membrane disruption…关于杀生物和细胞膜破坏的研究.pdfVIP

AKINPELU ET AL. Biol Res 42, 2009, 339-349 339 BR Biol Res 42: 339-349, 2009 Studies on the biocidal and cell membrane disruption potentials of stem bark extracts of Afzelia africana (Smith) 1, 2 1 1, * DAVID A AKINPELU , AYOBAMI O AIYEGORO and ANTHONY I OKOH 1 Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Alice 5700, South Africa. 2 Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Nigeria. ABSTRACT We had recently reported antibacterial activity in the crude extract of the stem bark of Afzelia africana (Akinpelu et al., 2008). In this study, we assessed the biocidal and cell membrane disruption potentials of fractions obtained from the crude extract of the plant. The aqueous (AQ) and butanol (BL) fractions exhibited appreciable antibacterial activities against the test bacteria. The minimum inhibitory concentrations of the AQ and BL fractions ranged between 0.313 and 2.5 mg/ml, while their minimum bactericidal concentrations varied between 0.625 and 5.0 mg/ml. Also, the AQ fraction killed about 95.8% of E. coli cells within 105 min at a concentration of 5 mg/ml, while about 99.1% of Bacillus pumilus cells were killed by this fraction at the same concentration and exposure time. A similar trend was observed for the BL fraction. At a concentration of 5 mg/ml, the butanol fraction leaked 9.8 μg/ml of proteins from E. coli cells within 3 h, while the aqueous fraction leaked 6.5 μg/ml of proteins from the same organisms at the same concentration and exposure time. We propose that the stem bark of Afzelia africana is a potential source of bioactive c