食品质量与安全专业论文 大米样品中黄曲霉毒素的检测.docVIP

食品质量与安全专业论文--大米样品中黄曲霉毒素的检测 Rice samples in the detection of aflatoxin 专业名称:食品质量与安全 答辩时间:2012年6月 摘 要 大米,是稻子的子实脱过壳而成的。大米是中国人的主食之一,无论是家庭用餐还是去餐馆,米饭都是必不可少的。糙米中的无机盐、B族维生素(特别是维生素B1)、膳食纤维含量都较精米中的高。大米是提供B族维生素的主要来源,是预防脚气病、消除口腔炎症的重要食疗资源。米粥具有补脾、和胃、清肺功效。米汤有益气、养阴、润燥的功能,能刺激胃液的分泌,有助于消化,并对脂肪的吸收有促进作用。自从1960年发现黄曲霉毒素AFT后,大米食品一直是卫生监测的重点。 本研究主要是通过利用ELISA方法,检测大米农作物是否被黄曲霉菌寄生产生黄曲霉毒素所感染。探讨了ELISA测定食品样品过程中提取液pH值、含盐量、金属离子(铁,铜,铅,砷)含量对检测结果的影响,初步证实了影响的存在,同时用一定的溶剂萃取提取液可消除这些影响,为建立不同食品样品中黄曲霉毒素B1的提取方法提供了理论依据。 关键词:黄曲霉毒素;大米;检测;影响 Abstract Rice is the rice seeds, took off the shell into. Chinese rice was one of the staple, whether it is family dinner or go to a restaurant, the rice is essential. Brown rice in the inorganic salts, B vitamins especially vitamin B1 , dietary fiber content than rice in high. Rice is the main source of vitamins B, prevent beriberi, elimination of oral inflammation are important food resources. Rice porridge with spleen, stomach, lung function and. The soup Qi, Yin, moistening function, can stimulate the secretion of gastric juice, helps digestion, and promoted the absorption of fat. Since the 1960 discovery of aflatoxin AFT , food and health has been the focus of monitoring. This research is mainly through the use of ELISA method, detection of rice crop is yellow aspergillus infection, aflatoxin. On the determination of food samples in the process of ELISA extract pH, salinity, metals iron, copper, lead, arsenic content influence on the detection results, preliminary confirmed the influence of existence, at the same time with certain solvent extraction liquid can eliminate these effects, as established in different food samples of aflatoxin B1 extraction method provides theoretical basis. Key words: Aflatoxin;rice;detection;effects of 目 录 摘 要 I Abstract II 目 录 III 第一章 前 言 1 1.1课题研究的背景、目的 1 1.2大米的营养价值 2 1.3黄曲霉毒素的危害 2 1.3.1黄曲霉毒素(AFT) 2 1.3.2黄曲霉毒素B1 3 1.4大米中黄曲霉毒素检测方法综述 4 第二章 实验部分 6 2.1 ELISA检测食品中黄曲霉毒素B1 6 2.1.1设备与仪器 6 2.1.2样品采集 6 2.1.