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Article Physical Chemistry January 2011 Vol.56 No.1: 1–6 doi: ? SPECIAL TOPICS: Preparation of an anti-diethylstilbestrol monoclonal antibody and development of an indirect competitive ELISA for detection of diethylstilbestrol in animal tissue, urine and bile 1 2 3 3 2 1 LI WeiHua , MENG Meng , HE FangYang , WAN YuPing , XUE HuYin , LIU Wei , YIN 1 1 3 3 4 1* 2* WeiWei , XU Jing , FENG CaiWei , WANG ShanLiang , LU Xiao , LIU JinTing XI RiMo 1 School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China; 2 College of Pharmacy, Nankai University, Tianjin 300071, China; 3 Beijing Wanger Biotechnology Co., Ltd., Beijing 102206, China; 4 Central Laboratory of Shandong Academy of Agricultural Sciences, Jinan 250100, China Received April 22, 2010; accepted August 5, 2010 Based on the preparation of an anti-diethylstilbestrol (DES) monoclonal antibody, a simple and convenient indirect competitive enzyme-linked immunosorbent assay (ELISA) method for DES detection has been developed. The monoclonal antibody demon- strated high sensitivity to DES with an IC50 value of 275 pg mL–1 and detection limit (LOD) of 90 pg mL–1 . The specificity of the assay was studied by measuring cross-reactivity of the antibody with structurally related compounds of ethinyl estradiol (7%), estrone (0.1%), estriol (0.1%), and diethylstilbestrol benzoate (0.1%). Chicken, fish, shrimp, urine and bile spike