原创力文档A Genetic Linkage Map of Kenaf (Hibiscus cannabinus L.) Based on SRAP, ISSR and RAPD Markers推荐.pdfVIP
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A Genetic Linkage Map of Kenaf (Hibiscus cannabinus L.) Based on SRAP, ISSR and RAPD Markers推荐
Agricultural Sciences in China
2011, 10(9): 1346-1353 September 2011
A Genetic Linkage Map of Kenaf (Hibiscus cannabinus L.) Based on SRAP,
ISSR and RAPD Markers
1, 2 1, 3 1 1 4 1
ZHANG Guang-qing , QI Jian-min , ZHANG Xiao-chen , FNAG Ping-ping , SU Jian-guang , TAO Ai-fen ,
1 1, 3 5
LAN Tao , WU Wei-ren and LIU Ai-min
1 Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fuzhou 350002, P.R.China
2 Crop Sciences Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, P.R.China
3 College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China
4 Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, P.R.China
5 Department of Chemistry and Center for Metals in Biocatalysis , University of Minnesota, St. Paul, MN55108, USA
Abstract
Kenaf (Hibiscus cannabinus L.) is one of the most economically important crops for non-wood fiber production. The
objective of this study was to establish a genetic linkage map of kenaf with higher density of molecular markers. A semi-
wild variety Ga42 and a cultivar Alain kenaf were used as parents to construct an F2 population consisting of 155 plants.
The genetic linkage map comprising 134 marker loci was constructed, including 65 sequence-related amplified
polymorphism (SRAP), 56 inter-simple sequence repeat (ISSR), and 13 randomly amplified polymorphic DNA (RAPD)
markers. This map spans 2 108.9 cM and contains 20 linkage groups with an average marker density of 15.7 cM between
the adjacent markers.
Key words: kenaf, genetic map, SRAP, ISSR, R
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