Analysis of genetic diversity in Acorus calamus populations in South推荐.pdf

Biochemical Systematics and Ecology 40 (2012) 156–161 Contents lists available at SciVerse ScienceDirect Biochemical Systematics and Ecology journal homepage: www.elsevier .com/locate/biochemsyseco Analysis of genetic diversity in Acorus calamus populations in South and North East India using ISSR markers V.K. Abdul Kareem a, P.E. Rajasekharan a, *, B.S. Ravish a, S. Mini a, Anuradha Sane a, T. Vasantha Kumar b a Division of Plant Genetic Resources, Indian Institute of Horticultural Research, Hessaraghatta Lake P.O., Bangalore 560 089, India b Section of Medicinal Crops, Indian Institute of Horticultural Research, Hessaraghatta Lake P.O., Bangalore 560 089, India a r t i c l e i n f o a b s t r a c t Article history: Inter simple sequence repeat (ISSR) markers were used to estimate genetic diversity Received 13 April 2011 among 21 samples of Acorus calamus , an endangered medicinal herb from South and North Accepted 22 September 2011 East India. Out of 100 primers screened 14 generated distinct and reproducible DNA Available online 11 November 2011 fragments. Using these primers, 103 discernible DNA fragments were generated of which 53 (51.5%) were polymorphic and remaining 50 (48.5%) were monomorphic. The amplified Keywords: PCR fragment size ranged from 450 to 2250 bp. Nei’s genetic diversity (h) and Shannon’s Acorus calamus index (I ) among the population were estimated as 0.2005 and 0.2946 respectively. Value Clonal plant ISSR