Analysis of genetic variation in grass carp (Ctenopharyngodon idellus)推荐.pdf

Biochemical Systematics and Ecology 37 (2009) 549–555 Contents lists available at ScienceDirect Biochemical Systematics and Ecology journal homepage: www.elsevi /locate/biochemsyseco Analysis of genetic variation in grass carp (Ctenopharyngodon idellus) from native and colonized regions using ISSR markers Qin Chen a, Cheng-Hui Wang a, *, Guoqing Lu b, Xiao Song a, Jia-Wei Xu a, Qin-Ling Yang a, Si-Fa Li a a Key Laboratory of Aquatic Genetic Resources and Utilization, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China b Department of Biology, University of Nebraska at Omaha, Omaha, NE 68182, USA a r t i c l e i n f o a b s t r a c t Article history: The grass carp (Ctenopharyngodon idella), a freshwater species native to China, have been Received 15 May 2009 introduced in more than 100 countries. In this paper, inter simple sequence repeat (ISSR) Accepted 22 August 2009 markers were used to evaluate genetic variation within and among grass carp populations sampled from its native (China) and colonized habitats (USA, Hungary, and Japan). Twenty- Keywords: two ISSR primers were used to generate 207 bands, of which 82 (39.61 %) were Ctenopharyngodon idella polymorphic. The highest genetic diversity was observed in the Yangtze River population, Genetic diversity whereas the lowest diversity was found in the Tone River population (Japan). Compared Differentiation ISSR with colonized populations,