自动微流体系统实现多步磁生化分析外文文献.pdfVIP

Sensors and Actuators B 245 (2017) 477–483 Contents lists available at ScienceDirect Sensors and Actuators B: Chemical j o u r n a l h o m e p a ge : w w w. elsev /locate/snb Automatic microfluidic system to perform multi-step magneto-biochemical assays S. Gómez-de Pedro, M. Berenguel-Alonso, P. Couceiro, J. Alonso-Chamarro, M. Puyol ∗ Sensors Biosensors Group, Department of Chemistry, Autonomous University of Barcelona, Edifici Cn, 08193 Bellaterra, Catalonia, Spain a r t i c l e i n f o a b s t r a c t Article history: A simple and automatic microfluidic system to perform a multistep colorimetric magnetic bead-based Received 3 October 2016 enzyme-linked oligonucleotide sandwich assay in a short analysis time is reported. As a proof of con- Received in revised form cept of the advantages of miniaturizing and automating bio-chemical assays, the determination of an 20 December 2016 Escherichia coli oligonucleotide has been addressed. Moreover, the use of magnetic beads allows simpli- Accepted 25 January 2017 fication of the required cleaning steps as well as the application of an active mixing strategy, based on Available online 29 January 2017 magnetic beads manipulation by a magnetic actuator, which enhances the reagents mixture inside the microsystem. The presented microsystem allows reducing the analysis time to less than thirty minutes Keywords: