小型香猪组织工程皮肤移植后基底膜重建的实验研究文档.docVIP

小型香猪组织工程皮肤移植后基底膜重建的实验研究 2005年O7月第22卷第07期PmcJMedamp;Pharm.Vol22,2005-07No.07 小型香猪组织工程皮肤移植后 基底膜重建的实验研究术 吴国选①伍津津⑦朱堂友②鲁元刚②毕建军②沈利群① (0153医院皮肤科,河南郑州450042;②第三军医大学大坪医院皮肤科,重庆400042) [摘要】目的研究贵州小型香猪来源的组织工程皮肤自体及同种异体移植后基底膜形成情况,为复 合壳多糖组织工程皮肤临床应用提供实验依据.方法按我科已建立的方法构建猪的组织工程皮肤,然 后进行自体及同种异体移植实验,观察移植的猪组织工程皮肤的存活及创面愈合情况,并于移植后1,4,8 周取标本,行组织学检查和免疫组化染色,观察移植后猪组织工程皮肤基底膜形成情况.结果猪组织工 程皮肤自体及同种异体移植后均存活,创面达到理想的愈合效果,组织学检查显示表皮明显增厚,表皮突 明显,真皮胶原束显着增粗变密,新生血管网丰富.PAS染色基底膜带呈完整连续阳性.免疫组化染色显 示:表,真皮交界处层粘连蛋白抗体免疫组化染色阳性.结论我们构建的猪组织工程皮肤自体及同种异 体移植后,可获得理想的创面愈合效果,组织工程皮肤基底膜形成完整,与正常皮肤相似.本实验为复合 壳多糖组织工程皮肤临床应用提供了有力的实验依据. [关键词]组织工程皮肤自体移植异体移植基底膜 [中圈分类号】R318[文献标识码】A Exper~mentalstudyonreconstructionofbasementmembraneafterautograftandaUograftof swinecompositechitosan廿ssueengineeredskinWUGuoxuan~. WUJinjin,ZHUTangyou.eta1.a~Dept. efDermatology,No.153HospitalofPLA,Zhengzhou450042,Chma [Abstract】0bjecfiveToprovideexperimentMevidenceforclinicalapplicationofthecomposite chit~santissueengineeredskin,aswinetissueengineeredskinwasfabricatedtostudythereconstruction obloodvesselsafterautograftandallograft.MethodsSeriallypassagedcellswereusedtofabricate t}jcompositechitosantissueengineeredskin.Thenthetissueengineeredskinwereautograftedand allograftedontofullthicknessexcisedwoundsoftwoswinesafterorganotypiccultureinvitro.Itsviability andwoundshealingstatuswereobserved.Biopsyofthegraftstakenat1st,4thand8thweekrespectively wereexaminedbyhistologicallyandimmunohistochemicallytoinvestigatereconstructionofthebasement membraneofthetissueengineeredskin.ResultsThetissueengineeredskinwerelivingafterautograft andallograftwiththesurroundingnormalskinoftheswine.Thewoundshealedwel1.Thetissueengineered skintransplantedontotheswineshowedimmunohistochemicallythatlamininWtt8expressed.Conclusion Woundsmaybehealedwellafterautograftandallograft.Thebasementmembraneoftissueengineeredskin iswel1.Thisanimalstudyprovidesexperimentalevidenceforclinicalapplicationofthecompositechitosan tissueengineeredskin. eywords】TissueengineeredskinAutograftAllograftBasementmemb