人补体C3功能片段rC3B的克隆、表达和活性鉴定推荐.docVIP

人补体C3功能片段rC3B的克隆、表达和活性鉴定 人补体C3功能片段rC3B的克隆、表达和活性鉴定 【摘要】 本研究获取人类补体C3上与补体Ⅲ型受体in monocytes, the peptide rC3B, including the bindingsite, as expressed, purified and identified. rC3B, the binding part of human plement C3 to CRⅢ, as selected by puteraided modeling and summarizing researches published. Then, rC3B gene fragment as amplified by PCR, and cloned into prokaryotic vector pQE30a. The fusion protein rC3B as expressed in E.coli M15 and purified by Ni2+chelating affinity chromatography. The activity of rC3B as identified by Western blot and adherence assay ith monocytes. The results s