抗生素耐药Engineering a virus to select against antibiotic resistance.pptVIP

This is our plan of attack. First we outlined our circuit and used computational modeling to refine our design. Then we constructed multiple versions of the circuit and characterized them in plasmids. We plan on using the data from this characterization to further refine our modeling and design until the circuit is fully functional. Now Thomas will explain our circuit design and construction. Time (min) Excitation = 514 nm, Emission = 527 nm In contrast, EYFP is produced under similar conditions in TcS E. coli TcS+circuit TcS-circuit [aTc] = 1 μM KD = 0.01nM Biochemistry. Vol. 35:23 p.7439