(精选)【医学课件课件大全】 蛋白质电泳教学课件.pptVIP

蛋白质电泳;原 理 ;电泳原理示意图;在蛋白质组学中对电泳的分类;一维电泳;凝胶浓度和蛋白质分离范围;缓冲液的选择;12%胶常用的低分子量标准蛋白;双向电泳(2DE);2DE仪器系统;2DE在蛋白质组学方案中所处的位置;双向电泳的操作程序(以进行差别表达蛋白组分析为例);目前，普遍采用预制的固定pH梯度胶条(immobilized pH gradient strips, IPG strips)。商品化的IPG strips可以从Amersham Pharmacia公司或BioRad公司购买。;IPG胶条制备(casting of IPG strips);;Procedure; Assembly of the gel casting cassette ;IPG gel casting ;The acidic, dense solution is pipetted into the mixing chamber and the basic, light solution into the reservoir of the gradient mixer, an extra portion of the dense solution is prepared and pipetted into the mold prior to pouring the gradient. ;After