SEROLOGY MANUAL - Welcome - Mount Sinai Hospital —血清学检查手册-欢迎-西奈山医院—.doc

Microbiology Department Policy Procedure Manual Policy # MI/MD/v13 Page 1 of 2 Section: Molecular Diagnostic Tests Manual Subject Title: Table of Contents Issued by: LABORATORY MANAGER Original Date: June 08, 2007 Approved by: Laboratory Director Revision Date: July 31, 2013 Annual Review Date: July 31, 2013 MOLECULAR DIAGNOSTIC TESTS MANUAL TABLE OF CONTENTS Chlamydia trachomatis and Neisseria gonorrhoeae Molecular Testing 3 Cepheid Xpert C. difficile Assay 15 Hepatitis B (HBV) DNA Quantitative Testing 29 Hepatitis C RNA PCR 36 Roche cobas? 4800 Human Paillomavirus (HPV) Test 43 Nucleic Acid Extraction –Biomerieux NucliSENS easyMAG 54 Nucleic Acid Extraction for Whole Blood – Biomerieux NucliSENS easyMAG 62 BK Virus DNA Detection 70 Cytomegalovirus DNA Detection 79 Epstein Barr Virus DNA Detection 89 Enterovirus RNA Amplification and Detection Procedures 98 Herpes simplex Virus/Varicella-Zoster Virus DNA Detection 108 Influenza A/B RSV PCR by Focus Diagnostics Simplexa? 119 Parvovirus B19 DNA PCR 125 Respiratory Virus Panel (RVP) Multiplex PCR (Luminex) 135 WNV PCR by ALTONA KIT v. 1.0 and ROTORGENE 144 Roche TaqScreen MPX Assay and West Nile Virus Assay 155 APPENDIX I - Molecular Diagnostic Tests Schedule 164 APPENDIX II – Influenza A, B and H1N1 Virus rPCR back up testing by RotorGene 165 Appendix III - Respiratory Synctial Virus (RSV) PCR backup testing by RotorGene 176 APPENDIX V - General PCR Precautions and Decontamination Procedures 186 General PCR Precautions 186 Decontamination Procedure 187 WNV Decontamination Procedure 187  Microbiology Department Policy Procedure Manual Policy # MI/MD/v12 Page 2 of 2 Section: Molecular Diagnostic Tests Manual Subject Title: Table of Contents APPENDIX VI – Virology Specimen Accessioning Guide 189 Appendix VII: Creating Standard Curve for Quantitative PCRs 190 Appendix VIII - Instructions for Pipetting by epMOTION 192 Instructions for Pipetting One Test on epMOTION 192 Instruction For Pip