Modulation of Radiation induced Biochemical Changes in Testis of 调制的辐射引起的睾丸组织中的生化变化.docVIP

Modulation of Radiation Induced Biochemical Changes in Cerebrum of Swiss Albino Mice by Grewia asiatica Muktika Ahaskar1, K.V. Sharma1, Smita Singh1, Rashmi Sisodia1* 1Radiation Cancer Biology Laboratory, Department of Zoology, University of Rajasthan, Jaipur, India-302004 Running title: Grewia asiatica against radiation in Cerebrum * corresponding author Dr. Rashmi Sisodia , 6A Maharani college staff quarters, Near Diggi House Jaipur, Rajasthan, India-302001 Tel.: +91-141-2370880; fax: +91-141-2701137 e-mail : rashsisodia@yahoo.co.in Abstract: The aim of the present study was to evaluate the radioprotective effect of Grewia asiatica fruit pulp extract (GAE) on Swiss albino mice exposed to gamma radiation. In the present study radioprotective efficacy of Grewia asiatica (rich in anthocyanin, carotenes, Vit.C etc.) was studied against radiation induced biochemical alterations in mice cerebrum. For experimental study, healthy Swiss Albino mice were selected from an inbred colony and divided into four groups. Group I (normal) did not receive any treatment. Group II was orally supplemented (GAE) once daily at the dose of 700 mg / Kg.b.wt / day for fifteen consecutive days. Group III (control) received distilled water orally equivalent to GAE for fifteen days than exposed to 5 Gy of gamma radiation. Group IV (drug + IR) was administered orally (GAE) for 15 consecutive days once daily before exposed to single dose of 5Gy of gamma radiation respectively. Mice were sacrificed at different autopsy intervals viz. 1, 3, 7, 15 and 30 days and cerebrum were removed for various biochemical estimations viz. glutathione (GSH), lipid peroxidation (LPO), protein. GAE pre-treatment renders protection against various biochemical changes in mice cerebrum. Radiation induced augmentation in the levels of LPO was significantly ameliorated by GAE pretreatment. Radiation–induced depletion in the level of GSH, protein was checked significantly by GAE administration. Key words: Gr