瘦素促进骨髓间充质干细胞成骨分化并增加内植物.docVIP

瘦素促进骨髓间充质干细胞并 李亘 贺韬 李业 上海交通大学医学院附属仁济医院骨科,上海 200127 ：（BMSCs）对 方法：骨髓腔骨髓间充质干细胞，后，使用在骨髓间充质干细胞中μg/kg,20μg/kg），在手术后4处死，。 ：骨髓间充质干细胞动物实验中，与组（μg/kg）μg/kg处理组 结论：骨髓间充质干细胞并且 关键词瘦素 通讯作者： 电子邮箱为：dyqrjgk@126. Leptin promotes BMSCs osteogenic differentiation and increases bone formation in plant – bone interface model Li Gen,He Tao, ZHANG Chao,Li Ye,Song Hui,DONG Yuqi Department of Orthopedics, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai,China 200127, Abstract: Objictive Research on the role of leptin promoting BMSCs osteogenic differentiation and increasing bone formation in plant – bone interface model. Methods：After obtain from SD rats and identified by flow cytometry, BMSCs were cultured osteogenic liquid with different concentrations of leptin (0ng / ml, 10ng / ml, 20ng / ml) for 21days,then expression of ALP, RUNX2 was detected . New Zealand rabbits were injected subcutaneously with different doses of leptin (0μg/kg, 20ug / kg) from plant - bone interface bone formation model and reared for 28days,and bone formation were detected. Results：ompared to the control,cultured in containing different concentrations of leptin (10ng / ml, 20ng / ml) osteogenic liquid, BMSCs were detected increased ALP, RUNX-2 expression with Western-blot ,and the result was statistically significant. Compared to the control, bone formation increased in plant - bone interface bone formation model from New Zealand rabbits injected subcutaneously with 20ug / kg doses of leptin. Conclusion：promotes BMSCs osteogenic differentiation and increases bone formation in plant – bone interface model Key words : Leptin ; BMSCs ;Osteogenic ; A plant - bone interface bone formation model 钛金属及钛合金材料内植物被广泛地应用于矫形外科、口腔种植体、骨缺损修复以及关节置换等医学领域。其中钛合金材料作为医用生物金属内植物的最重要组成部分之一，其在 如何增强其生物活性促进内植物-骨界面牢固固定的。因此本关注并 1 材料和方法： 1.1主要试剂： DMEM培养基(低糖)、胎牛血清、青霉素、胰蛋白酶、链霉素购于Gibco公司；40 %丙 酰胺溶液、PVDF膜、West Dura Extended Duration Substrate购于美国 Pierce 公司； PageRulerTM Plus Prestained Protein Ladder (10-250 kDa) 购于Ferme