蓝氏贾第鞭毛虫类高尔基体的研究_朱艳红.pdfVIP

672 Chinese Journal of Zoonoses 2007, 23 (7) : 1002- 2694( 2007) 07- 0672- 03 蓝氏贾第鞭毛虫类高尔基体的研究 1 1 1 2 3 朱艳红 , 雷清华 , 彭 挺 , 牛安欧 , 卢思奇 : 探讨类高尔基体结构在蓝氏贾第鞭毛虫滋养体和成囊不同时段的存在情况及 动态变化机理 用可特异性标记真核细胞高尔基体的荧光染料C6- NBD 神经酰胺, 标记有活性的蓝氏贾第鞭毛虫滋养体及成囊61218 h 虫 体, 并用荧光显微镜观察同时利用半定量RT- CR 方法检测高尔基体膜结合蛋白golgin245 和膜泡融合的调节蛋白Rab1 在蓝氏贾第鞭毛虫对数生长期滋养体及成囊61224 h 的虫体中的表达水平 极少数蓝氏贾第鞭毛虫滋养体被C6- NBD 神经酰胺标记; 多数处于成囊61218 h 时段的虫体均可见被特异标记成亮绿色的核周围区域golgin245 和Rab1 在滋 养体及成囊诱导61224 h 的虫体中的表达水平无显著变化 蓝氏贾第鞭毛虫在成囊过程中出现类高尔基体结构, 而 在对数期滋养体中却没有在蓝氏贾第鞭毛虫成囊过程中, 高尔基体结构成分无大量合成, 出现的类高尔基体结构可能是各 组分临时组装的结果 : 蓝氏贾第鞭毛虫; 类高尔基体; C6-NBD 神经酰胺; 半定量RT- CR : R382. 2 : A Observations on the Golgis body-like structure in Giard ia lamblia ZHU Yan-hong, LEI Qing-hua, ENG Ting, NIU An-ou, LU S-i qi (Dep artment of Biology , Tongj iMedical College, H uaz hong University of Science and Technology , uhan 430030, China) ABSTRACT:To explore the existence and dynamic changes of the Golgi body-like structure in the log-phase and encysting trophozoites ( 6, 12, 18 hours) of Giardia lamblia, these trophozoites w ere stained w ith C6-NBD ceramide, a specific marker for Golgi body in eukaryotic cells, and then they w ere observed w ith fluorescence microscopy. The transcript expressions of a membrane-combined protein golgin-245 and vesticular traffic protein Rab- 1 of Golgi body were analyzed in log-phase and encys- t ing trophozoites ( 6, 12, 24 hours) w ith RT- CR assay. It w as found that only few log- phase trophozoites were labeled by means of C6-NBD-ceramide, however, most of t he encysting trophozoites were labeled in perinuclear region. No change on the transcript expression levels of golgin 245 and Rab- 1 protein w as detected during growth and encystation of Golgi body. F