何宇霆-流动注射分光光度法检测皮革和制革废水中的甲醛.doc

流动注射分光光度法检测皮革和制革废水中的甲醛 专业： 轻化工程 学生 何宇霆 指导教师 张新申 摘要：游离甲醛不管在空气中还是在水体中对环境都有很大破坏。常规的检测方法都要使用昂贵且携带不便的分析仪器，分析成本较高并且不能实现在线检测。本文针对现有方法的不足，建立了一种灵敏度高、操作相对简单、可用于现场测定制革废水及皮革中甲醛的自动分析方法。工作原理为：乙酰丙酮在乙酸铵-乙酸缓冲溶液中与甲醛及氨发生反应，生成微黄色的2,6-二甲基-3,5-二乙酰基-1,4-二氢吡啶，该产物在414nm处有最大吸收，根据在该波长处的吸光度与甲醛浓度成比例关系对甲醛进行定量分析。据此，建立了流动注射分光光度法测定皮革和制革废水中甲醛的方法。在最佳条件下，甲醛的浓度在0.025～3.000mg/L具有良好的线性关系，相关系数0.9996，检出限为0.025mg/L，回收率为96.41％～99.58％，用1.00mg/L标准溶液进样分析的相对标准偏差为0.95％(n=10)。利用此方法对实际皮革废水样品及绵羊皮革样品中甲醛进行测定，都取得了满意的结果。 关键词：流动注射；分光光度法；皮革；甲醛；制革废水 Flow Injection Spectrophotometry Determination of Formaldehyde in Leather and Leather Waste Water Major: Light Chemical Engineering Undergraduate He Yuting Supervisor Zhang Xinshen Abstract: Free formaldehyde which is in air or water has a destructive effect on environment. Usual detection methods have several defects such as using costliness and cumbersome analytical apparatus, high cost and having a difficult to realize on-line detection. In this paper, we apply flow infection principle to set up a highly sensitive and easily handled way to determine formaldehyde in the leather and leather waste water at crime scene. The main content of research above is described as follows: Acetyl acetone had a reaction with formaldehyde and ammonia in the buffer solution of ammonium acetate and acetic acid to form primrose 3,5-diacetyl-2,6- dimethyl-1,4-diludine.The absorbance of this product was measured spectrophotometrically at 390 nm. According to the proportional relationship between absorbency in this wavelength and concentration of formaldehyde, formaldehyde was analyzed quantitatively. Then flow injection spectrophotometry determination of formaldehyde in leather and leather waste water was set up. The linearity concentration range of the calculation curve was from 0.025-3.000mg/L, and correlation coefficient was 0.9996, the detect limit was 0.025mg/L, percent recovery was 96.41%~99.58%, and the relative standard deviation was 0.95% (n=10) obtain