二化螟抗药性监测方法及相对敏感基线南京农业大学学报.PDFVIP

南京农业大学学报 　2000 , 23 (4) : 25～28 　 Journal of Nanjing Agricultural University 二化螟抗药性监测方法及相对敏感基线 陈长琨 　李秀峰 　韩召军 　李国清 　王荫长 (南京农业大学农业部病虫监测与治理重点开放实验室 , 南京 210095) 摘要 　选用安徽太湖县二化螟 ( Chilo suppressalis Walker) 自然种群, 用 6 种药剂对 6 龄幼虫进行毒力测定 , 并与国内外已 知敏感品系的LD50值比较 , 在证实了该种群具有较高的敏感水平后 , 经室内纯化和连续饲养近20 代 , 育成相对敏感品系 (THS) 。为提高试验准确性 , 简化操作 , 使用 4 龄幼虫进行抗药性研究。用于测试的 4 龄幼虫形态特征为头宽 (086 ± 004) mm , 体重 (85 ±15) mg , 前胸与头宽之比 119 ±004 , 体长 ( 100 ±08) mm 。在详细观察4 龄幼虫发育历期和 形态特征后 , 选用该龄幼虫 , 建立了包括沙蚕毒素、有机磷、杂环类和生物制剂等类的 6 种杀虫剂的相对敏感基线。 关键词 　二化螟 ; 抗性监测 ; 相对敏感基线 分类号 　S4351121 Method for monitoring insecticide resistance in rice stem borer ( Chilo suppressalis Walker) and relative susceptible baselines data Chen Changkun , Li Xiufeng , Han Zhaojun , Li Guoqing and Wang Yinchang ( Key Laboratory of Monitoring and Management of Plant Disease and Insects , Ministry of Agriculture , Nanjing Agric Univ , Nanjing 210095) ABSTRACT 　Susceptible rice stem borer was collected from Taihu County , Anhui Province , and continuously reared free from insecticide in laboratory for 20 generations. After the susceptibility was reconfirmed by bioassay with 6 conventional insecticides and comparison with baseline data reported previously , a susceptible strain was developed and the larvae of different instar were used to study and modify the topical treatment method for practical use in resistance monitoring. It was found that 4th instar larvae were more suitable. Bulk of uniform 4th instar larvae (big enough to handle) , was easier to obtained with short rearing time , less food materials and labor consumption. The test