病毒感染的诊断.pptVIP

culture cells (normal cells) Cytopathic effect (CPE)an observable morphologic changes which take place in the host cells as a result of virus replication respiratory syncytial virus CPE herpes simplex virusCPE CPE of poliovirus PLAQUE ASSAYplaque forming unit, PFU hemadsorption Inclusion body Virus-specific structure that produced in the course of viral multiplication in the host cell. Diagnostic aid Viral diagnosis with EM Detection of viral protein Enzyme-linked immunoassay (ELISA) Immunofluorescent assay (IFA) Sandrich ELISA Immunofluorescent assay (IFA) Serologic diagnosis Hemagglutination inhibition test Hemagglutination inhibition test serologic diagnosis Western blot (WB),Immunoblot ELISA Enzyme linked immunosorbent Assay (70 %) Herpesvirus, Hepatitis virus IF Immunofluorescence Parvovirus B19, EBV, Hantanvirus CBR Complement binding reaction Respiratory Infection, RSV, Influenza HIT Hemagglutination inhibition test Rotavirus WB Western blot, Immunoblot HIV, HCV, EBV NT Neutralization test Enterovirus * Diagnosis of Viral Infection isolation of virus detection of virion detecting virus with EM detection of Viral proteins（antigen） component nucleic acid serologic diagnosis：specific antibodies in sera Cell culture: primary cell semi-continuous cell continuous cell Embryonated eggs Inoculation in animals isolation of virus CPE Inclusion body hemadsorption Chorioallantoic membrane epithelial epithelioid fibroblastic uninfected early infection late infection Adenovirus CPE uninfected syncytium uninfected early infection late infection uninfected early infection late infection cytomegalovirus, in an owl’s eye appearance, intranuclear An intracytoplasmic and intranuclear acidophilic