uplc-tof-ms法鉴定胀果甘草药渣中黄酮类成分.pdfVIP

• 558 • 现代药物与临床 Drugs Clinic 第 27 卷 第 6 期 2012 年 11 月 UPLC-TOF-MS 法鉴定胀果甘草药渣中黄酮类成分 1 2 2* 1 1 1 1* 张 娟 ，刘 芬 ，李 宁 ，卿德刚 ，孙 宇 ，贾晓光 ，倪 慧 1. 新疆维吾尔自治区中药民族药研究所，新疆 乌鲁木齐 830002 2. 沈阳药科大学 中药学院，辽宁 沈阳 110016 摘 要：目的 建立胀果甘草药渣中黄酮类成分的超高效液相色谱–高分辨飞行时间质谱（UPLC-TOF-MS ）定性分析方法。 方法 Agilent SB-C18 柱（100 mm×4.6 mm ，1.8 μm ）；流动相乙腈–0.1% 甲酸水溶液，梯度洗脱；体积流量0.4 mL/min ；柱 温 25 ℃；检测波长254 nm 。ESI 离子源，飞行时间质谱检测器。对比自制对照品进行鉴别。结果 共鉴定出 8 个黄酮类成 分，分别为 2,4,4-三羟基查耳酮、甘草查耳酮 D 、甘草查耳酮甲、4-羟基-2,2-二甲基吡喃[5,6,6,7]黄酮、甘草黄酮 C、光 甘草酮、甘草黄酮 B 和 kanzonol E 。结论 建立了一种简单、可靠的 UPLC-TOF-MS 方法对胀果甘草药渣中黄酮类成分进行 了鉴定，对胀果甘草药渣综合利用有一定参考价值。 关键词：胀果甘草药渣；黄酮；2,4,4-三羟基查耳酮；甘草查耳酮 D ；甘草查耳酮甲；超高效液相色谱–高分辨飞行时间质 谱（UPLC-TOF-MS ） 中图分类号：R284.21 文献标志码：A 文章编号：1674 - 5515(2012)06 - 0558 - 04 Identification of flavonoids gruffs of Glycyrrhiza inflate waste by UPLC-TOF-MS 1 2 2 1 1 1 1 ZHANG Juan , LIU Fen , LI Ning , QING De-gang , SUN Yu , JIA Xiao-guang , NI Hui 1. Xinjiang Institute of Chinese Materia Medica and Ethical Materia Medica, Urumqi 830002, China 2. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China Abstract: Objective To establish the identification method for flavonoids in gruffs of flavonoids in G. inflate by UPLC-TOF-MS. Methods The method of identification was performed on Agilent SB-C18 column (100 mm×4.6 mm, 1.8 μm), the mobile phase consisted of acetonitrile and 0.1% formic acid aqueous solution with gradient elution program. The flow rate was 0.40 mL/min, the column temperature was 25 ℃, and the wavelength was set at 254 nm. The detector was TOF Mass Spectrometer with an ESI ion source. The data were compared with those of the reference substances. Results Eight flavonoids were identified from the gruffs in G. inflate and were