成人感染性腹泻临床分析.docVIP

导致幼儿呕吐的病学张洪英南京市疾病预防控制中心（江苏，南京，210003）疾病预防控制中心（江苏，南京，2100）（江苏，南京，2100）【摘 要】目的 了解掌握的病学特点，为科学预防的流行提供科学依据。方法 收集2010年的例患者的。结果 结论 地区的病是应加强相应的抗病毒健康教育。 【关键词】 ；；病学； 中图分类号：R512.5 文献标识码：A 文章编号： ’s vomiting Zhang Hong-ying1, Qi Xian2, Hong Lei1, Hua Ling-hui3, Wu Bin2, Jiang Xiao1, He Min 1,Shi Li-min1 1 Center for Disease Control and Prevention of Nanjing, Nanjing 210003, China 2 Center for Disease Control and Prevention of Jiangsu Province, Nanjing 210009, China 3 Center for Disease Control and Prevention of Yuhuatai District, Nanjing 210009, China 【Abstract】Objective To the main reason for cases in one kindergarten with the sudden?symptoms of?vomiting so as to provide scientific reference for prevention and control of enteric infectious diseases. Methods The vomit of three children were collected during December 2010, treated and cultured for pathogenic bacteria detection as routines or detected by real-time PCR and sequencing for the viral detection. Results No pathogenic bacterium was detectable. Norovirus was detected in three vomit, with same subtype of G4-Ⅱ. The results confirmed the etiology is viral infection, but not bacteria food poisoning. Conclusion There is?the threat of?norovirus in Nanjing district. Norovirus, ?subtypeⅡ-G4, was the pathogen, resulting in the?vomiting cases?in the?kindergarten. It is the same subtype of norovirus as in other countries; and anti-virus?advocacy should be?strengthened to?health education and especially for protection of children. 【Key Words】 ; poisoning; Etiology; Norovirus 基金项目：江苏省卫生厅预防医学研究课题（编号：Y201024）南京市医学科技发展计划ZKX09033）。通讯作者：张洪英 E-mail, xiao99now@ 幼儿园见到的幼儿呕吐胃肠型感冒扁桃体炎引起的吃饭太急太饱。 1 材料与方法 资料 1.2 细菌学常规检测 3份呕吐物进行细菌的常规分离培养，目标细菌包括金黄色葡萄球菌、沙门杆菌、大肠杆菌、副溶血性弧菌、变形杆菌。具体操作方法参照GB-T 4789 2006 进行。 1.3 病毒学核酸检测 3份呕吐物同时进行荧光定量PCR检测2。引物和探针2见表1。同时设定阳性对照和阴性对照。阳性对照，本实验室制备；阴性对照为配置PCR体系所用的无DNA酶无RNA酶的无菌水。检测仪器为 ABI 7500 Real-Time PCR System，SDS v1.3.2，试剂盒为 TaqMan Probe Reaction Mix (ABI)，PCR扩增反应条件：50°30 min；95℃10min；95℃ 15 s，0℃ 30s，72℃1mi