BasicTutorial flow cytometry文档.pdfVIP

FlowJo Basic Tutorial FlowJo is software for the analysis of flow cytometry data. This tutorial will quickly introduce you to FlowJo using an example experiment, where a FITC anti-CD8 reagent is titrated to determine the most economical concentration for staining in future experiments. Table of Contents Introduction 1 Step 1: Load samples into the workspace 3 Step 2: Analyze the control sample in detail   7 Step 3: Copy gates and statistics to all samples 12 Step 4: Verify gates on all samples   13 Step 5: Generate a graphical report 17 Step 6: Generate a table of statistics   23 FlowJo is a product of Tree Star, Inc. www.FlowJ USA: (800) 366-6045 International: 1 541-201-0022 flowjo@ FlowJo was written by Adam Treister and Mario Roederer in 1996, based on concepts developed at the Herzenberg laboratory at Stanford. We are indebted to our active and enthusiastic users worldwide for their ideas, discussions and tireless testing of new versions. FlowJo, its tutorials, documentation and web site are Copyright © Tree Star, Inc. 1997-2009. All Rights Reserved. • FlowJo Basic Tutorial • July 2009 - V.7.5 • © MMIX• Basic Tutorial Introduction FlowJo software is used for the analysis of flow cytometry data. You can use FlowJo to analyze all of your flow cytometry data - regardless of the cytometer used to collect your data files. This tutorial will introduce you to FlowJo and