同种异体幼犬睾丸组织块大网膜移植研究.PDF

·20· ·论著· 同种异体幼犬睾丸组织块大网膜移植研究 孙杰 施诚仁 张忠德 李怀远 沈 萍 【摘要】目的通过研究同种异体幼犬睾丸组织块大网膜移植，探讨同种异体睾丸组织块移植对男 童原发性性腺功能低下症的治疗意义。方法以幼犬为实验对象，采用异体睾丸组织块移植的方法， 将供体动物的部分睾丸组织种植到受体动物．并经皮下隧道延伸到阴囊的大网膜上。术后每周测定受 体血清睾酮含量，连续测量4次。将移植组织切片分析，HE染色观察组织结构。结果睾丸组织块移 植后，受体动物血清睾酮水平逐渐上升，至手术后第4周，体内激素含量较移植前明显升高(P0．05)。 病理观察移植的睾丸组织块在受体大网膜上成活。可见到间质细胞形态。结论异体睾丸组织块大网 膜移植是可行的，治疗的目的仅限于提高体内雄激素水平．不涉及生育。 【关键词】移植，同种；睾丸／移植；网膜／移植；狗 of oftestis on canines’omentum．SUN船，SHI Studyallotransplantationfragmentsjuvenile Cheng-ren, ZHANG Medical Childrens 200127,China Zhong-de,etal，DepartmentofSurgery,ShanghaiCenter,Shanghai To thecurative of oftestis to 【Abstract】0bjeetlve significanceallotransplantationfragments study male Juvenilecanineswere as primaryhypogonadism．Methods employedexperimentalobjectives．Partial of hadbeen testisthedonorswas tothe omentumwhich extendedtothescrotumsub-· transplantedreceptors7 testosteroneconcentrationwasdetected for4times were cutaneously．Serum weekly postoperatively．Grafts observed withHE Thelevelofthe testosteroneincrease microscopicallystaining．．Results receptors’serum 4weeks The testis weresur- graduall)；which fragments improvedsignificantlypostoperationtransplanted be rivedintheonlentuIllandtestlcuhr couldidentified mesenchymal qL￡ydig)cells microscopically．Con- dusions oftestis onomentumcanbe aimofthe is Allotransplantationfragments performed．Theexperiment