NREL法测定大米草原料组分的含量.PDFVIP

第37 卷第5 期 Vol.37 No.5 井冈山大学学报( 自然科学版) 2016 年9 月 Sept. 2016 Journal of Jinggangshan University (Natural Science) 43 井冈山大学学报( 自然科学版) 43 文章编号：1674-8085(20 16)05-0043-04 NREL 法测定大米草原料组分的含量 * 1,2 2 1,2 谢茹胜 ，刘 健 ，龙敏南 (1.福建生物工程职业技术学院，福建，福州 350002 ；2.厦门大学能源学院，福建，厦门 36 1102） 摘 要：采用美国国家可再生能源实验室(NREL)方法定量大米草原料中纤维素、半纤维素及木质素。72 ％浓硫酸 水解1 h、4 ％稀硫酸水解1 h 可将大米草的纤维素、半纤维素降解为可用HPLC 定量的单糖，适宜的 品添加量 为0.3 g 。同时，NREL 法测定大米草纤维素、半纤维素及木质素的含量分别为32.92 ％、27.65％和24.2％。这三 个组分的含量 评价大米草预处理、酶解及发酵工艺条件的重要依据。 关键词：NREL 法；纤维素；半纤维素；大米草；定量分析 中图分类号：O657.7 文献标识码：A DOI:10.3969/j .issn.1674-8085.2016.05.009 DETERMINATION OF SPARTINA ANGLICA STOCK COMPONENTS BY NREL METHOD * 1,2 2 1,2 XIE Ru-sheng , LIU Jian , LONG Min-nan (1. Fuji an Vocational College of Bioengineering, Fuzhou, Fuji an 350002, China; 2. College of Energy, Xiamen University, Xiamen, Fuji an 36 1100, China ） Abstract: Cellulose, hemicellulose and lignin of Spartina anglica were quantified by National Renewable Energy Lab (NREL) method. It was showed that the cellulose and semicellulose of S. anglica could be degraded to mono-sugars which could be quantified by HPLC after it was hydrolyzed with 72 ％ concentrated sulphuric acid for 1 h and 4 ％ dilute sulphuric acid for 1h with the appropriate additive amount of 0.3 g sample. Meanwhile, the contents of cellulose, hemicellulose and lignin of S. anglica determined by NREL method were 32.92 ％, 27.65 ％ and 24.2 ％ respectively. The contents of these three fractions are importan