Graves眼病IgG和细胞因子IFN—γ.docVIP

Graves眼病IgG和细胞因子IFN—γ 中国医科大学JChinMedUniv第28卷第1期1999年2月 , . Graves眼病IgG和细胞因子IFN一对体外培养 人眶后成纤细胞的刺激作用7 坠主一圭三.苎滕卫平李静李忠民.. (中国医科大学第一临床学院眼科.沈陌ll000I) 摘要目的:Graves眼病(GO)的病理组织学特点是球后成纤维细胞产生大量葡萄糖胺聚糖(GAGs)和胶原蛋 白沉积,造成眼球竞出.末文从体外实验的角度探讨末病对球后成纤堆细胞的刺激因素.方法:应用10例Graves眼 稿,7删Graves病,4俐结带性甲状臃肿伴甲状朦机能亢进症病人和8删正常^IgG和细胞因子IFN—与培养的 ^日匡后成蚌堆细胞(RF)在体外孵育,观察透明质醢(HA),IV型腔原蛋白产生量和细胞增殖的状态.结果:GO和 其他甲状腺疾病病人以及正常凡的IgG都能明显刺激RF台成HA增加,奋蛆之间无明显差异}对Ⅳ型腔原蛋白 的台成和RF增殖,各组IgG皆无影响F细胞因子IFN—能够刺斑RF合成HA明显增加,但是却押割IV型腔原蛋 白的合成.结论:Graves眼病患者血清中未盅现针对眶后成纤维细胞的特异性自身抗体;IFN一刺激RF产生HA 的作用可能与末病病理变化有关工拿竹 关键词Grave$艰病F眶后成纤维细胞}免疫球蛋白G{干扰素{遣明质畦 ,————～——————～ StimulatingEffectsofIgGfromthePatientswithGravesOphthalmopathy andlFN—yon目uma廿RetroorbRalFjbroblastsinVitro ChenLei,LiYushu.TengWeiping,etal (Deparm~entofOphthalmology,TheFirstClinicalCollegetChinaMedicalUniversity,Shenyang?110001 ABSTRACT0bjecttve:Gravesophthalmopathy(GO)ischaracterizedhistologicallybyallaccumulationof glycosaminoglycansandcollagenproducedfromretroorbitalfibrohlasts(RFthatresultsinexophthalmos?Inpre— sentstudy.thestimulatingfactorstoRFinvitroforthepatientswithGOweinvestigated.MethodsIThepuri— fledIgGfrom10patientswithGravesophthalmopathy,7patientswithGravesdisease?4patientswithtoxic muhinodulargoiterand8normalpersonsandeytokineIFN-TwmincubatedwithhumanRFinvitroandthesyn— thesis0{hyaluronieacid(HA)andtype1VcollagenandRFproliferationweredetectced.R~ults:All0{theIgG fromthepatientswithGOandotherthyroiddiseaseandnormalpersonscouldstimulateRFwithincreaseoiHA synthesis.buttherewerenosignificantdifferenceamongthem?IFN一couldincreasethesynthesisofHAinthe humanRF,whereasinhibitedthesynthesiso{DnpeIVcollagen.Conclusion{Nostimulatingantibodiesspecifically directedtoRFinthepatientswithGOwerefoundinthisstudy.Theef{ectso{IFN一onthesynthesisofHApro ducedinRFmightplayo.13importantroleinpathogellesisinGO. KEYWORDSGravesophthalmopathylretroorbitalfibroblasts;inter{eron-V;hyaluronlcacid Graves眼病(G0)的病理特点是球后结缔组织 内大量葡萄糖胺聚糖(Glyoosaminoglyoans,GAGs) 和胶原蛋白沉积.亲水的粘多糖导致球后组织水肿. 已知眶后成纤维细胞(Retroorbit