生物矿化与仿生材料Biomim07SynFerr.pptVIP

Application of Biongenic Materials:Synthesis of Colloids in Ferritin Last lecture: classification of synthetic strategies outlook next lectures “Flat Pearls” as example for the application of living organisms Today: repetition: stability of colloids synthesis of colloids in ferritin Literature: Trevor Douglas ?Biomimetic Synthesis of Nanoscale Particles in Organized Protein Cages“ in: S.Mann (Ed.) “Biomimetic materials chemistry” (VCH, Weinheim 1996). Repetition: Stability of ColloidsExample: Growth and Condensation of SiO2-Partikels Hydrolysis and condensation of Si-alkoxides Possibilities for growth: growth of particles by addition of monomers particle aggregation Colloids: particles with narrow size distribution on nm-scale Repetition: Stability of ColloidsInteractions van-der-Waals interactions dipole-dipole permanent-permanent Keesom permanent-induced Debye ?transitory-transitory“ London ? attractive forces on the nm-scale Electrostatic interactions M-OH + H+ M-OH2+ M-OH + OH- M-O- + H2O ? generally repulsive forces, but... ? at point of zero chargeno electrostatic repulsion ? pH-dependency Repetition: Stability of ColloidsElectrostatic Stabilization Stern-Layer: “tightly” associated layer of hydrated counterions Helmholtz-Plane: interface between Stern-Layer and ... Gouy-Layer: counterion diffusion not hindered ?Slip-plane“: interface of hydrate shell which is effective during electrophoresis Repetition: Stability of ColloidsSteric Stabilization Causes: entropic, mobility of polymer strands is reduced energetic, solvent molecules adsorbed to polymer have to be desorbed Further possibility for stabilization: synthesis of colloid particles in protein cages Ferritin shell built up by 12 antiparallel pairs of H- and L-chains (=24 subunits) quaternary structure does form spontaneously in solution different possibilities for bio-mimetic materials synthesis Possible Reaction Pathways Enzymatic Control (in vivo) maximum control by cooperative