p53和顺铂对wnt通路抑制剂frphe的表达作用.pdfVIP

15 卷 5 期 生命科学研究 灾燥造援员5 晕燥援5 圆园11 年 10 月 蕴蚤枣藻 杂糟蚤藻灶糟藻 砸藻泽藻葬则糟澡 Oct. 圆园11 p 53 顺铂对Wnt 通路抑制剂Frp HE 的表达作用 1 2 1 蕾 , 班 武 , 任青青 (1. 南方医科大学南方医院药学部, 中国广东广州510515; 2. 广州军区总医院, 中国广东广州5100 10) 摘 要 将携带p 53 基因的复制缺陷型腺病毒载体A dp53 导入到p 53 完全缺失的人肝癌细胞株中, 培养人肿 瘤细胞, 并分别加入5 滋mol/L 顺铂(Pt)作用24 h, 以RT PCR 技术检测Frp HE mRNA 的表达作用, 以流式细胞 术检测A dp 53 的转基因情况和茁鄄catenin 的改变为功能指标评价Wnt 通路的变化. Frp HE mRNA 表达水平在 转染A dp 53 和作用Pt 24 h 后即有明显升高, 在人大肠癌细胞和人神经胶质瘤细胞中, 未见Frp HE mRNA 表 达. 茁鄄catenin 表达水平逐渐下降. 提示外源性物质p 53 和顺铂能明显诱导Frp HE 的表达, 进而产生抑制Wnt 通路的作用. 关键词: A dp 53; 顺铂; Frp HE; Wnt 通路; 茁鄄catenin 中图分类号: R394.6; Q756 文献标识码: A 文章编号: 1007 7847(2011)05 04 15 05 Regulation of p 53 and Cisplatin on Expression of Wnt Signaling Inhibitor Frp HE 1 2 1 LA Lei , BAN Wu , REN Qing qing (1. Dep artment of Pharmacy, Nanf ang Hosp ital, Southern Medical Unive rs ity, Guangz hou 5 10515, Guangdong, China; 2. Guangz hou General Hosp ital of Guangz hou Military Command, Guangz hou 510010, Guangdong, China) Abstract ：Human hepatocarcinoma cells were transfected with a replication defective advenovirus encoding (A dp53), human tumor cells were transfected with antitumor drug cisplatin after 24 h, A dp53 and catenin expression was measured by fluorescence activated cell sorting (FACS) and then Wnt signaling pathway inhibitor Frp HE expression was detected by RT PCR. Frp HE mRNA was initially potentiated by A dp53 and cisplatin after 24 h, no inhibitor Frp HE was expressed in Lovo and