高效液相色谱法测定白葡萄汁中苯甲酰脲类农药.pdfVIP

※分析检测 食品科学 2013, Vol.34, No.10 169 高效液相色谱法测定白葡萄汁中苯甲酰脲类农药 周建科，韩朝家，唐翠苓，付艳芳 (河北大学理化分析中心，河北省分析科学技术重点实验室，河北 保定 07 1002) 摘  要：以二氯甲烷为萃取剂，甲醇为分散剂，采用分散液液微萃取-正相高效液相色谱法测定白葡萄汁中4种 V V 苯甲酰脲类农药(氟苯脲、杀铃脲、除虫脲、氟铃脲) 。色谱条件：Apollo Silica柱；正己烷-无水乙醇(97 :3 ， / ) 为流动相；流速：1.0mL/min ；紫外检测波长：200nm 。在0.33～100μg/mL范围内4组分线性良好，方法检出限在 5.80～6.60ng/mL之间，平均回收率范围是73.72%～97.85%，相对标准偏差范围是1.33%～4.62% 。该方法快速、检 出限低、重现性好，可用于白葡萄汁中苯甲酰脲类农药的测定。 关键词：高效液相色谱法；苯甲酰脲；液液分散微萃取；白葡萄汁 Determination of Benzoylurea Insecticides in White Grape Juice by High Performance Liquid Chromatography ZHOU Jian-ke ，HAN Chao-j ia，TANG Cui-ling，FU Yan-fang (Research Center of Physics and Chemistry Analysis, Key Laboratory of Analytical Science and Technology of Hebei Province, Hebei University, Baoding 07 1002, China) Abstract ：Residues of benzoylurea insecticides (teflubenzuron, triflumuron, diflubenzuron and hexaflumuron) in white grape j uice were determined by dispersive liquid-liquid micro-extraction combined with normal-phase high performance liquid chromatography. In the dispersive liquid-liquid micro-extraction, the extraction solvent was methylene chloride and dispersive solvent was methanol. The analytes were separated on Apollo Silica column with a mobile phase made up of n V V -hexane and anhydrous alcohol (97:3, / ) at a flow rate of 1.0 mL/min and detected at 200 nm. Good linearity was found in the range of 0.33 to 100 μg/mL with correlation coefficients above 0.999. The limits of detection were between 5.80 ng/mL and 6.60 ng/mL. The average recoveries of the benzoylurea insec