Cytochrome P450 Electron Flow细胞色素P450电子流.pptVIP

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2018-04-17 发布于江西
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Cytochrome P450 Electron Flow细胞色素P450电子流.ppt

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Cytochrome P450 Electron Flow细胞色素P450电子流.ppt

C C 12 13 C C 12 13 2 NADH + 2H+ 2 NAD+ Cytochrome b5ox Cytochrome b5red Cytochrome b5 reductasered Cytochrome b5 reductaseox desaturaseox desaturasered O2 2 H2O ER Desaturase Electron Flow NAD(P)H NAD(P) Cytochrome b5ox Cytochrome b5red Cytochrome P450 reductasered Cytochrome P450 reductaseox Cytochrome P450red Cytochrome P450ox Substrate Substrate OH O O2 H2O Cytochrome P450 Electron Flow Acknowledgements Major Advisor: Dr. David Hildebrand Committee Members: Drs. Michael Barrett and Maelor Davies Dr. Sergei Avdiushko Dr. David McClure Hangsik Moon Greg Budziszewski Cunxi Wang Charles McCracken Dr. Hiro Fukushige Elena Dimuzio Product of enzymatic reaction C C C C OH C C O C C O 12 13 12 13 12 13 12 13 linoleic acid ricinoleic acid vernolic acid vernolic acid Substrate oleoyl-PC oleoyl-PC linoleoyl-PC linoleoyl-PC Reductant NAD(P)H NAD(P)H NADPH 10X NADH NAD(P)H ???? Desaturase ???? Hydroxylase E. lagascae Epoxygenase V. galamensis Epoxygenase Enzyme Product Inhibition in the presence of cyanide yes yes yes no Enzyme Properties ???? Desaturase ???? Hydroxylase E. lagascae Epoxygenase V. galamensis Epoxygenase Enzyme Fe Moiety non-heme iron (diiron cluster) non-heme iron (diiron cluster) heme iron ? Reductase cytochrome b5 reductase cytochrome b5 reductase ? Cytochrome b5 reductase or cytochrome P450 reductase Product inhibition in presence of carbon monoxide no no yes yes Product inhibition in the presence of anti- cytochrome P450 reductase antibodies no no yes no Product inhibition in the presence of anti- cytochrome b5 reductase antibodies yes yes yes yes ???? Desaturase ???? Hydroxylase E. lagascae epoxygenase V. galamensis epoxygnease Enzyme Product inhibtion in the presence of carbon monoxide no no yes yes Product inhibition in the presence of anti- cytochrome P450 reductase antibodies no no yes no Product inhibition in the presence of anti- cytochrome b5 reductase antibodies yes yes yes yes ???? Desatu