大肠杆菌o111引起的溶血性尿毒症hemolyticuremicsyndromehus.pptVIP

Chapter 27 Foodborne Gastroenteritis Caused by Escherichia coli Tsuei-Yun Fang 節錄自Modern Food Microbiology, 2005 Jay, J. M., 7th ed. SEROLOGICAL CLASSIFICATION E. coli ? over 200 recognized O serotypes ? more heterogeneous carbohydrate side chains of O antigen Flagellar proteins are less heterogeneous ? Fewer H antigenic types THE RECOGNIZED VIRULENCE GROUPS Five virulence groups of E. coli are recognized: enteroaggregative (EAggEC) enterohemorrhagic (EHEC) enteroinvasive (EIEC) enteropathogenic (EPEC) enterotoxigenic (ETEC). ? Food infection EHEC and ETEC : Toxin-mediated infection Enteroaggregative E. coli aggregative adherence a 60-Mda plasmid is need for the production of fimbriae and outer membrane protein (OMP) The distinguishing clinical feature of EAggEC strains is a persistent diarrhea that lasts 14 days, especially in children. Enterohemorrhagic E. coli Similar to EPEC in the possession of the chromosomal gene eae A (encodes the intimin protein that is essential for A/E ) the intimin protein is a virulence factor (adhesin) of EPEC and EHEC the production of attachment-effacement (A/E) lesions Enterohemorrhagic E. coli Disimilar to EPEC in affect only the large intestine (in piglet models) produce large quantities of Shiga-like toxins (SLT, Stx) EHECs produce fimbriae by a 60-Mda plasmid ? attach to culture cells E. coli O157:H7 The Toxins of EHEC Shigella dysenteriae produces a potent toxin? Shiga toxin (Stx) The toxins of EHEC strains of E. coli have been referred as Shiga-like toxin ? SLT-1 and SLT-2 ? Stx1 and Stx2 ? new terminology The Toxins of EHEC Stx1 differ from Shiga-toxin (Stx) by three nucleotides and one amino acid, and neutralized by antibodies to Stx. Toxin gene is chromosomal. Stx-sensitive cells possess a toxin receptor. The Toxins of EHEC The mechanisms of Stx, Stx1, and Stx2 are the same. They are N-glycosidase that cleave a specific adenine residue from the 28S subunit of eukaryotic rRNA, leading to the inhibition of pr