地衣型真菌的i型内含子及其在系统发育中的应用.pdfVIP

Mycosystema 菌 物 学 报 15 November 2011, 30(6): 920-931 jwxt@ ISSN1672-6472 CN11-5180Q ©2011 Institute of Microbiology, CAS, all rights reserved. 地衣型真菌的 I 型内含子及其在系统发育中的应用 1,2 1,3 1* 1* 曹叔楠 刘萌 周启明 郭守玉 1 中国科学院微生物研究所真菌地衣系统学重点实验室 北京 100101 2 中国科学院研究生院 北京 100039 3 山东农业大学生命科学学院 泰安 271018 摘 要：以蜈蚣衣属、黑蜈蚣衣属地衣样品为材料，结合 GenBank 中相关数据，对地衣型真菌核糖体小亚基 DNA 上的 I 型内含子分布模式进行归纳，并探讨了其在地衣型真菌系统发育研究中的应用。结果表明在地衣型真菌核糖体小亚基 DNA 上存在多个 I 型内含子插入位点，通过二级结构分析给出了天然状态下 I 型内含子发生转座的证据。分析显示，I 型内含子 作为分子标记，只适合用于种下单位的系统发育研究中。 关键词： Ⅰ型内含子分布模式，二级结构，小亚基rDNA ，ITS 序列 Group I introns in lichen forming fungi and their application for phylogenetic analysis CAO Shu-Nan1, 2 LIU Meng1, 3 ZHOU Qi-Ming1* GUO Shou-Yu 1* 1Key Laboratory of Systematic Mycology and Lichenology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China 2 Graduate University of Chinese Academy of Sciences, Beijing 100039, China 3College of Life Sciences, Shandong Agriculture University, Tai’an 271018, China Abstract: Group I introns in SSU (Small Subunit) rDNA of lichen forming fungi and their distribution modes were analyzed in this study. The data were obtained from eight species of the lichen genera Physcia and Phaeophyscia with some sequences retrieved from GenBank. Our result indicates that there are multiple group I introns insertion sites in SSU rDNA of lichen forming fungi, and their secondary structures gives the evidences of transposition when group I introns are in its nature states. Our result also demonstrates that group I introns are only suitable for the phylogenetic analysis at infraspecific levels. Key words: distribution mode of group I introns, secondary structure, small subunit rDNA, ITS sequence 基金项目：国家自然科学基金（Nos.） * Correspon