Englishpaper教程.pptVIP

本周五全天 （上午9：30——下午8：00） 地点：实验楼735 辅导时间 Course objectives Basically, learn and remember some professional words and expressions of plant pathology; Being capable to read professional English papers; Share your scientific idea with other people in English. Course instructor Guo, Jian-Hua （郭坚华） Tel and Fax:(office) (lab) (home) Email: jhguo@njau.edu.cn MATERIALS AND METHODS 材料与方法 MATERIALS AND METHODS 按照内容分成多个小标题，小标题不分级。 一个标题下的具体内容按照操作的顺序叙述。 一般使用被动语态，有时为了行文活泼也使 用主动语态。 注意动词的用法： 一般使用过去时， 避免多次重复同一个动词， 准确使用中文翻译相同但英文意义不同的动词。 MATERIALS AND METHODS Bacterial strains. Plant sources. Isolation of endophytic bacteria. Bacterial growth conditions. Phenotypic characterization of bacterial isolates. Inoculation of plants with bacterial endophytes. Isolation of endophytic bacteria from experimentally inoculated plants. Growth room studies. Homologous and host range greenhouse studies. （同源性和寄主范围的温室研究） Dent corn greenhouse studies and field trials. Statistical analysis. 16S rRNA gene amplification and sequencing. Nucleotide sequence accession numbers.? Bacteria were grown to the mid-log phase, pelleted by centrifugation (3,440 g, 10 min, 4°C), washed twice, and suspended in phosphate buffer (recite after class). 细菌生长到对数生长期的中期， 3,440 g, 4°C条件下离心10 min，在磷酸缓冲液中洗两次并悬浮其中。 Inoculation of plants with bacterial endophytes. To confirm inoculation density and purity, an aliquot of each culture was serially diluted in phosphate buffer and plated on NBY medium. 为了验证接种浓度和纯度，每份培养物被磷酸缓冲液倍比稀释并涂于NBY培养基。 Corn and sorghum plants were inoculated in triplicate when a minimum height of 7.6 cm was reached and the stalks were at least 0.5 cm in diameter, which corresponded to 7 to 14 days after seed germination. 当玉米和高粱株高7.6 cm 以上，茎粗至少0.5 cm 时（对应于出芽后生长7-14天），接种细菌，重复三次。 For the initial time point (1 day postinoculation), whole-plant samples of corn and sorghum were obtained as described above. For subsequent sample