口腔生物学精品教学（中山大学）7.细胞培养.pptVIP

Applications of cell culture in dental researches Oral biology and Pathology Jingjing Quan (BDS, MSc, PhD) Guanghua School of Stomatology Sun Yat-sen University Objectives to definite the method of cell culture and how to use it for studies in vitro to introduce several kinds of cell types used in dental researches to understand the concept of tissue engineering and stem cells to explain other research assays commonly applied in oral biology Part 1 Cell culture the removal of cells from animals and plants and their subsequent growth in a favorable artificial environment the major tool providing model systems to study the natural environment in vivo Adherent Cells Suspended Cells Adherent cells cells have to be grown on a suitable substrate specifically treated to allow cell adhesion epithelial-like cells (polygonal in shape, regular dimensions, epithelial cells) fibroblast-like cells (multipolar, elongated shape, mesenchymal cells) Suspended cells cells adapt to suspension culture without attaching to a surface free-floating in culture medium lymphoblast-like (sphere in shape, cells derived from blood, macrophages) Cell culture growth Immortalization the ability to live forever, an immunity from?death to designate cells expressed the telomere-lengthening?enzyme telomerase, and thereby avoided?apoptosis can be created by induction of?oncogenes or loss of?tumor suppressor genes Proliferation of cell passage Cell culture conditions non-contaminated environment suitable vessel of substrate gas (O2, CO2) and pH medium supplies essential nutrients temperature and osmotic pressure Non-contaminated environment Cell culture hood Substrate cell culture vessels flasks; petri-dishes; roller bottles; multi-well plates Physical-chemical environment 95% air, 5% CO2, humid pH 7.2-7.4 temperature（36.5±0.5）℃ osmotic pressure 290～320 mOsm/kg Culture medium Cell culture technique contamination prevention sterile work